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分子生物学研究技能 | 关于植物激素研究那些事?Abscisic Acid (ABA)(建议收藏)

分子生物学研究技能 | 关于植物激素研究那些事?Abscisic Acid (ABA)(建议收藏)

2024-08-28 10:41

结合大家感兴趣的科研问题,接着给大家介绍植物激素。本期明星是脱落酸(Abscisic Acid (ABA)),也是最早研究的五种植物激素之一。结合相关资料一块学习下(敲黑板!!!)


Phytohormones – old timers and newcomers (5+4)


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脱落酸(ABA)


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ABA controls many plant processes including stress responses, development and reproduction


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ABA is synthesized in the plastid and cytoplasm and is derived from zeaxanthin, a plant pigment


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NCEDs are part of a large family, but only some are involved in ABA synthesis


Cluster of NCED genes thought to be involved in ABA synthesis


Some are primarily expressed in seeds, and others in vegetative tissues


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Transporters enhance ABA movement across membranes


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Other putative ABA transporters have been identified


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Perception and Signaling



The PYR/RCAR ABA receptors made Science magazines Top 10 list


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There are many genes encoding PYR/RCARs


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Different PYR/RCARs affect ABA responses slightly differently


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PYR/RCAR receptors bind ABA in a complex with ABI1 or other PP2Cs


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ABA / PYR1 binding sequesters PP2C and permits SnRK2 activity


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Thermal imaging can be used to monitor guard cell responses


Mutants impaired in ABA synthesis (aba2-13) or signaling (ost1-4) show reduced stomatal closure in response to a decrease in humidity, and so are cooler than wild-type plants


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Francis Darwin recognized stomatal effects on leaf temperature (1904)


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ABA signaling contributed to evolution of drought tolerance in land plants


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Transcription factors are major targets of CDPKs and SnRK2s


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ABA induces genes that protect cells from desiccation damage


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ABA controls seed maturation, dormancy and desiccation


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ABA - summary


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Conclusions and future directions - Synthesis and Transport


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参考文献:


1. Ma, Y., Szostkiewicz, I., Korte, A., Moes, D.l., Yang, Y., Christmann, A., and Grill, E. (2009). Regulators of PP2C phosphatase activity function as abscisic acid sensors. Science 324: 1064-1068.


2. Klingler, J.P., Batelli, G., and Zhu, J.-K. ABA receptors: the START of a new paradigm in phytohormone signalling. J. Exp.Bot. 61: 3199-3210.


3. Raghavendra, A.S., Gonugunta, V.K., Christmann, A., and Grill, E. (2010) ABA perception and signalling. Trends Plant Sci. 15: 395-401.


4. Gonzalez-Guzman, M., Pizzio, G.A., Antoni, R., Vera-Sirera, F., Merilo, E., Bassel, G.W., Fernández, M.A., Holdsworth, M.J., Perez-Amador, M.A., Kollist, H. and Rodriguez, P.L. (2012). Arabidopsis PYR/PYL/RCAR receptors play a major role in quantitative regulation of stomatal aperture and transcriptional response to abscisic acid. Plant Cell 24: 2483–2496.


5. Introduction to Phytohormones, The Plant Cell, Volume 22, Issue 3, March 2010, Page 1, https://doi.org/10.1105/tpc.110.tt0310.